The very best of Australian comedy come together to not laugh. Sam Simmons, Ed Kavalee, Anne Edmonds, Joel Creasey, Nazeem Hussain, Susie Youssef, Nick Cody, Dilruk Jayasinha, Becky Lucas and Frank Woodley enter the competition. Host Rebel Wilson explains the rules of the game - laughter is fatal. The moment a player cracks a chuckle, giggle, smirk or laugh, he or she will receive a yellow card violation. The second time he or she cracks, it's game-over for that player. As a tactic to eliminate fellow players, each comedian is given a single Joker Card that they can activate at any time. As soon as the comedian pulls it out, the entire room must devote their full attention for 3 minutes. After the initial pleasantries wane, the comedians go for the jugular. There are rapid-fire exchanges of verbal and physical humour, a surprise birthday cake, a pig's head and much more. Comedians are on the brink of laughter and then Sam Simmons shocks everyone with an unforgettable dance wearing a...

Our first comedian drops their guard and earns themselves a yellow card violations. With five hours on the clock, comedians bring out their best props, musical instruments and costumes. A comedic stunt becomes a fizzy ritual. When Rebel hands out her first red card it proves that in this game a simple one-liner can be fatal.

Now at the halfway point, the comedians are definitely feeling the heat. There is no shortage of yellow and red cards and the attacks mount up. From cultural cringe to fake boobs, foreskins and STDs, a unique charity challenge and lessons in multi-lingual sharting - nothing is sacred, and nothing is safe for our intrepid comedians. This is especially clear when a new character named Aunt Carol enters the room.

Rebel warns players against passive play and encourages the comedians to go on the attack. One comedian in particular tensely awaits the severity of his violation - will it be a yellow card or a red card?

Rebel hands out the red card no-one saw coming, Three comedians remain. With the last hour upon them, the final three make desperate attempts to knock each other out. They throw their dignity aside and not even grandmothers are off-limits. As the clock ticks down, Rebel takes matters into her own hands and launches a zombie attack. An eliminated player re-enters the game as a zombie and all hell breaks loose.

We're down to the final two. These two comedians pull out all the stops to defy mental and physical fatigue, while fighting to make the other laugh. It's a tour de force duel of wit, imagination and nonsense - via the unusual use of cleaning products, forceful comforting, recreations of sexual conquests and a good, salty cup of dick tea. Closing in on the finishing line, the confrontation transforms into a wild west gunfight, replacing pistols with...bum eggs? It's all fair game with $100,000 and the title of Last One Laughing on the line.